serolagic and microbiologic identification of avian mycoplasmas from slaughtered poultry in tehran

in this research sampling of poultry slaughterhouses in tehran province was done. the samples were investigated by serological tests : rapid serum agglutination (rsa) , elisa and broth & agar specific culture of mycoplasma. the main test for isolation and determination in this survey was rsa. in this investigation 4530 pharyngeal swab and blood sample from 226 poultryhouse were taken, in rsa method with mg antigen 11.27% were positive and false positive. this evaluation for ms was 38.19%. after this step the elisa test was done on the positive and false positive rsa results, and 66.86% of mg and 70.13% of ms were positive. the results indicated that in rsa method, false positive was high and specificity was low. comparison between prevalence of mg and ms in different seasons revealed in winter there are the most prevalence (p<0.001). out of 226 poultry house covered in the study, in 82 cases, mycoplasma was isolated from, which 36 cases was non mg and ms mycoplasma, 12 cases was mg, 29 cases was ms, and 5 cases was simultaneously mg and ms.